The flowers of Musa sapientum L. (MS) commonly known as ‘Banana Flowers’ are reported to be used in Indian folk medicine for the treatment of diabetes mellitus. But the probable mechanisms of antidiabetic activity are yet to be studied. The present study was designed to evaluate the antihyperglycemic activity of banana (Musa sapientum) flowers and development of suitable dosage form. The air dried powder sample of flower of Musa sapientum was extracted with ethanol and water by maceration method and subjected for phytochemical analysis. The extracts were analyzed by TLC which shown the presence of gallic acid. The quantitative estimation of gallic acid from extract was done by High Performance Thin Layer Chromatography (HPTLC). The antidiabetic potential of the extracts was tested using Oral Glucose Tolerance Test (OGTT) in normal rats. In OGTT, there was a significant reduction in glucose level of animals treated with aqueous and ethanolic extract at the dose of 200 mg/kg. In vitro assays were also performed for estimation of inhibition of α-glucosidase and α-amylase. IC50 values were calculated and inhibition efficiencies of aqueous and ethanolic extract were compared. The results have shown that both the extracts have good potential in management of diabetes. The human equivalent dose was calculated from OGTT results and tablet of 750 mg was formulated each containing 500mg aqueous extract of MS with optimized formula of excipients. The formulation was characterized and evaluated for physicochemical stability, and the gallic acid content in the table was assayed by HPTLC.
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